In Vitro Thrombolytic and Anti-Oxidant Activity Study of Abroma Augusta (Ulatkambal)

Antioxidants lavishly present in many natural source attributed their capability of slowing or preventing the oxidation of other molecules, helpful in the prevention of many age-related diseases and promotion of health. This study is designed to evaluate the antioxidant properties of the methanolic leaf extracts of Abroma augusta. After collection, drying, grinding and extraction, final extract of the plant sample were obtained through filtration followed by concentrated through evaporation in the water bath at 60ºC. DPPH (1,1-diphenyl-2- picryl hydrazyl) aid in the qualitative analysis of the antioxidant activity through color change from deep pink to pale yellow or yellow
upon spraying on the chromatogram of the Thin Layer Chromatography on which little amount of plant extract were applied before spraying. Inhibitory concentration 50% (IC50) value is a measure of the concentration of sample required to scavenge 50% of the DPPH free radicals helps in the quantitative study. Thrombolytic activity were utilized with the help of appendrof tube in which blood is kept and plant extract is applied whereas Streptokinase and distill water is used as positive and negative control. Inhibitory conc. 50% of the plant methanolic extract were found to be in the range of 35.87 % - 80.43% at different concentration of the plant extract which also show significant thrombolytic activity at variable range. The above result showed that the plant have good antioxidant and thrombolytic activity and hence support the acclaimed medicinal value of the crop.